Morphological types, fertility, and outcrossing rates were studied in a population
of 10 interspecific backcross progenies ( O. longistaminata/ O. sativa// O. sativa)
left under open pollination conditions. By segregation analysis at eight electrophoretic
loci, single-locus and multilocus estimates of the outcrossing rates were
calculated. In the first generation, 75% of the seeds came from outcrossing; this
rate decreased to 35% in the second generation, following pollen fertility restoration.
Outcrossing rates appeared primarily related to plant sterility and secondarily
to stigma length and exsertion. At the morphological level, an important diversity
of plant types was observed in the first generation, but plants were characterized
by various wild traits. The second generation spontaneously evolved toward a
more cultivated type, and transgressive segregants were observed for different
morphological traits. Allelic segregations at the F 1 level were normal, but the
second generation exhibited highly significant distortions. A loss of alleles coming
from the wild species was observed for 5 of the 8 loci and for all 10 families.
Oryza longistaminata is a wild species of rice that grows widely throughout intertropical
Africa. It covers a large range of ecological sites, from flooded plains to temporary
ponds, and propagates itself by developing vigorous rhizomes (Ghesquiere 1985). This
species is allogamous, with a self-incompatibility system, and shows the extreme
maximum values of stigma and anther length and number of pollen grains within the
Sativa species group (Oka and Morishima 1967).
This species shows significant diversity at the isozyme level (Ghesquiere 1988) and
appears to be among the most distant species from O. sativa within the Sativa group
(Second 1985). O. longistaminata has not intervened during the domestication of O.
sativa, nor in the latter’s diversification on the African continent since its introduction
there, because of the strong reproductive barrier that isolates the former from all other
species. This barrier is due to the action of two complementary lethal genes that cause
abortion of the embryo (Chu and Oka 1970a, Ghesquiere 1988). In spite of this barrier,
hybrid plants may be obtained, either by artificial crossing or, rarely, in seed sets
collected from wild plants along the borders of ricefields.